LOCUS       sequence-150240-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine ABC Transporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Johnson E, Nguyen PT, Yeates TO, Rees DC
  TITLE     Inward facing conformations of the MetNI methionine ABC transporter:
            Implications for the mechanism of transinhibition.
  JOURNAL   Protein Sci. 2012 Jan;21(1):84-96. doi: 10.1002/pro.765. Epub 2011 
            Dec 5.
  PUBMED    22095702
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.765"; 
            journalName: "Protein Sci"; date: "2012-01"; volume: "21"; issue: 
            "1"; pages: "84-96"
COMMENT     Sequence Label: MetNI (E166Q in MetN)
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(146..164)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      160..207
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    268..292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             307..329
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             342..371
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             387..401
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ttgctggtca ttctggttta tttaattcag ttcgcaggcg accgcatcgt ccgggctgtc
       61 actcgcaagt gagagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      121 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      181 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccag atctcgatcc
      241 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      301 aataattttg tttaacttta agaaggagat ataccatggg ccatcatcat catcatcatc
      361 atcatcatca cagcagcggc catatcgacg acgacgacaa gcatatgata aaactttcga
      421 atatcaccaa agtgttccac cagggcaccc gcaccatcca ggcgttgaac aacgtcagcc
      481 tgcatgtgcc agctggacaa atttatggcg ttatcggtgc ctcaggcgcg ggtaagagta
      541 cgcttatacg ttgtgtaaac ctgctggagc gcccaaccga gggtagcgtg ctggtcgatg
      601 gccaggaact gaccacgctg tcagaatccg agttgaccaa agctcgccgc cagattggta
      661 tgattttcca gcattttaac ctgctctctt cgcgtactgt ttttggcaac gtggctctgc
      721 cgctggagct ggacaacaca ccgaaagacg aggtcaaacg tcgcgtgacg gaattgctgt
      781 cattggttgg tcttggcgat aagcatgata gctacccgtc gaatctttcc ggtgggcaga
      841 aacaacgtgt ggcaattgcc cgtgcgttag ccagcaatcc caaagtattg ctgtgtgatc
      901 aagccaccag cgcgctggac ccggcaacga cacgttctat tctcgaactg ctgaaagaca
      961 tcaaccgccg tctggg
//
