LOCUS       sequence-150241-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pU6-Mb-crRNA
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     122..141
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(377..394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(628..647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
       61 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      121 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      181 gtggaaagga cgaaacaccg aatttctact gtttgtagat tgagacggga tccatccgtc
      241 tccttttttc tagattcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      301 tagttgtctt cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      721 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      961 ggtggcctaa ctacggctac actagaag
//
