LOCUS       sequence-150242-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S
  TITLE     Multiplex gene editing of the Yarrowia lipolytica genome using the 
            CRISPR-Cas9 system.
  JOURNAL   J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 
            10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
  PUBMED    27349768
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10295-016-1789-8"; journalName: "J Ind Microbiol 
            Biotechnol"; date: "2016-08"; volume: "43"; issue: "8"; pages: 
            "1085-93"
COMMENT     Sequence Label: pCAS1yl
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(23..59)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     primer_bind     complement(627..643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(627..643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(640..662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    651..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 accgatggcg tcctgatcca gtgacgagct tactcgtttc gtcctcacgg actcatcaga
       61 ctggactgcg gttagtactg caaaaagtgc tggtcggatg acgtggcgtc ttgtgtcgat
      121 gggaatggga ccacacccgg ccgtgtgctc aaagcccgtg gcaattgctg ctgcctctga
      181 aactcaccat tttgaatgat tcttatactc agaaggaaat gcttaacgat ttcgggtgtg
      241 agttgacaag gagagagaga aaagaagagg aaaggtaatt cggggacggt ggtcttttat
      301 acccttggct aaagtcccaa ccacaaagca aaaaaatttt cagtagtcta ttttgcgtcc
      361 ggcatgggtt acccggatgg ccagacaaag aaactagtac aaagtctgaa caagcgtaga
      421 ttccagactg cagtacccta cgcccttaac ggcaagtgtg ggaaccgggg gaggtttgat
      481 atgtggggtg aagggggctc tcgccggggt tgggcccgct actgggtcaa tttggggtca
      541 attggggcaa ttggggctgt tttttgggac acaaatacgc cgccaacccg gtctctgttt
      601 aaacaaacaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      661 ctcacaattc cacacaacat acgagccgga
//
