LOCUS       sequence-150245-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S
  TITLE     Multiplex gene editing of the Yarrowia lipolytica genome using the 
            CRISPR-Cas9 system.
  JOURNAL   J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 
            10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
  PUBMED    27349768
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10295-016-1789-8"; journalName: "J Ind Microbiol 
            Biotechnol"; date: "2016-08"; volume: "43"; issue: "8"; pages: 
            "1085-93"
COMMENT     Sequence Label: pCAS1yl
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttcctcttc ttcttggggt cagccctgct gtctccaccg agctgagaga ggtcgattct
       61 tgtttcatag agccccgtaa ttgactgatg aatcagtgtg gcgtccagga cctcctttgt
      121 agaggtgtac cgctttctgt ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc
      181 caagttggtc agagtaaaca agtggataat gttttctgcc tgctccctga tgggcttatc
      241 cctgtgctta ttgtaagcag aaagcacctt atcgaggtta gcgtcggcga ggatcactct
      301 tttggagaat tcgcttattt gctcgatgat ctcatcaagg tagtgtttgt gttgttccac
      361 gaacagctgc ttctgctcat tatcttcggg agaccctttg agcttttcat agtggctggc
      421 cagatacaag aaattaacgt atttagaggg cagtgccagc tcgttacctt tctgcagctc
      481 gcccgcacta gcgagcattc gtttccggcc gttttcaagc tcaaagagag agtacttggg
      541 aagcttaatg atgaggtctt ttttgacctc tttatatcct ttcgcctcga gaaagtcgat
      601 ggggtttttt tcgaagcttg atcgctccat gattgtgatg cccagcagtt ccttgacgct
      661 tttgagtttt ttagacttcc ctttctccac tttggccaca accagtacac tgtaagcgac
      721 tgtaggagaa tcgaatccgc cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag
      781 cttgtcgctg ttccttttcg ggaggatact ttccttggag aagcctccgg tctgtacttc
//
