LOCUS       sequence-150254-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine ABC Transporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC
  TITLE     The high-affinity E. coli methionine ABC transporter: structure and 
            allosteric regulation.
  JOURNAL   Science. 2008 Jul 11;321(5886):250-3. doi: 10.1126/science.1157987.
  PUBMED    18621668
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-07-11- 11"; volume: "321"; 
            issue: "5886"; pages: "250-3"
COMMENT     Sequence Label: MetNI (Lm)
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(58..63,64..80,81..86)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(261..308)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     304..322
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg
       61 acagcttatc atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag
      121 tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
      181 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatccg
      241 gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
      301 tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta
      361 gcagccggat cctcgagtca gacataaccc agtacctcta cttttacatg gtgttcctgc
      421 agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg
      481 ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt gttgacgttg
      541 aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga
      601 cgcagcatcg gcacgcagtc agtaaatggc tccgcttgca gacgttcctg gtaatcttcc
      661 gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt tttcggatgc
      721 gagaacactt cacttaccgt gtcctgctcg atcagttctc cattgctgat gaccgccacg
      781 caatcacaaa tgcgcttcac aacgtccatt tcgtgggtga tcaacagaat cgtcaacccc
      841 agacggcggt tgatgtcttt cagcagttcg agaatagaac gtgtcgttgc cgggtccagc
      901 gcgctggtgg cttcatcaca cagcaatact ttgggattgc tggctaa
//
