LOCUS       sequence-150255-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-CC3AI
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     437..458
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(516..537)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(777..796)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag attctagagc tagcgaattg ccaccatgta cccctacgac gtgcccgact
      121 acgccatcaa gatcgccacc aggaagtacc tgggcaagca gaacgtgtac gacatcggcg
      181 tggagaggga ccacaacttc gccctgaaga acggcttcat cgccagcaac tgcttcaacg
      241 aattcatgga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
      301 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      361 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
      421 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcgacgagg
      481 tggacggcga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      541 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      601 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      661 ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      721 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      781 acggcaacta caagacccgc cgcc
//
