LOCUS       sequence-150257-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine ABC Transporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC
  TITLE     The high-affinity E. coli methionine ABC transporter: structure and 
            allosteric regulation.
  JOURNAL   Science. 2008 Jul 11;321(5886):250-3. doi: 10.1126/science.1157987.
  PUBMED    18621668
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-07-11- 11"; volume: "321"; 
            issue: "5886"; pages: "250-3"
COMMENT     Sequence Label: MetNI (Lm)
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(141..159)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      155..202
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     244..263
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        244..262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    263..287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             302..324
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             337..366
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             382..396
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggtcattctg gtttatttaa ttcagttcgc aggcgaccgc atcgtccggg ctgtcactcg
       61 caagtgagag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      121 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      181 acgggtcttg aggggttttt tgctgaaagg aggaactata tccagatctc gatcccgcga
      241 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      301 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatcatcat
      361 catcacagca gcggccatat cgacgacgac gacaagcata tgataaaact ttcgaatatc
      421 accaaagtgt tccaccaggg cacccgcacc atccaggcgt tgaacaacgt cagcctgcat
      481 gtgccagctg gacaaattta tggcgttatc ggtgcctcag gcgcgggtaa gagtacgctt
      541 atacgttgtg taaacctgct ggagcgccca accgagggta gcgtgctggt cgatggccag
      601 gaactgacca cgctgtcaga atccgagttg accaaagctc gccgccagat tggtatgatt
      661 ttccagcatt ttaacctgct ctcttcgcgt actgtttttg gcaacgtggc tctgccgctg
      721 gagctggaca acacaccgaa agacgaggtc aaacgtcgcg tgacggaatt gctgtcattg
      781 gttggtcttg gcgataagca tgatagctac ccgtcgaatc tttccggtgg gcagaaacaa
      841 cgtgtggcaa ttgcccgtgc gttagccagc aatcccaaag tattgctgtg tgatgaagcc
      901 accagcgcgc tggacccggc aacgacacgt tctattctcg aactgctgaa agacatcaac
      961 cgccgtctgg ggttgacgat tctgttgatc acccacgaaa tggacgttgt gaagcgcatt
     1021 tgtgattgcg tggcggtcat ca
//
