LOCUS       sequence-150287-         435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt tctctgggta atcagcttgg
       61 cgttcagcag ctgccgccag tagttcttca tcttcttcac gacctcttcg gagggcacgt
      121 tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac cttgttgtcg atggagtcgt
      181 cggccagaaa gctctgaggc acgatatggt ccacatcgta gtcggacagc cggttgatgt
      241 ccagttcctg gtccacgtac atatcccgcc cattctgcag gtagtacagg tacagcttct
      301 cgttctgcag ctgggtgttt tccacggggt gttctttcag gatctggctg cccagctctt
      361 tgatgccctc ttcgatccgc ttcattctct cgcggctgtt cttctgtccc ttctgggtgg
      421 tctggttctc tctgg
//
