LOCUS       sequence-150288-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum
            ATCC 824.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren 
            X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
  TITLE     CRISPR-based genome editing and expression control systems in 
            Clostridium acetobutylicum and Clostridium beijerinckii.
  JOURNAL   Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
  PUBMED    27213844
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2016 May 23. doi: 10.1002/biot.201600053."
COMMENT     Sequence Label: pNICKclos1.0
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..72)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
ORIGIN
        1 atttctagct ctaaaaccag cctgtgattt aagtataact agtattatac ctaggactga
       61 gctagctgtc aactgcagcc aagcttaatc gcatttcata gattgacctc ccaataacta
      121 cgtggtgtta ttgggaggtc aatctatttc atttgcctct tgctcaaagt tcccaaattc
      181 gagtaagagg tatttttgtt tttggtcgtc gcctctcatt agtagttcag ggtttaacat
      241 taatactcca gtttttcttt ttataatatt tccttcttct aagattttaa gtgttgttat
      301 tactgtttgt agacttgttc ctgtagcttt tgctatttct cttgttgtag ctatcattgt
      361 attgttactt aagtggacat tatctaggat atagttaacg attttaagtt tttttccgcc
      421 aatcatatct aacatactta ttaattgcac tatatatgcc tttacgaagt taccagacgt
      481 ttgtttacgg tataacttgt ctacctctat gacttctcca ctttcttcgt ctatgagcct
      541 ctgagagcct ttatagactg ttccatatct ttctttcatc tttttctcac tccttatttt
      601 aaactattct aactatatca taactgttct aaaaaaaaaa gaacatttgt taaaagaaat
      661 tagaacaaaa tgagtgaaaa attagaacaa acaaattcct tataaacctt atcatctcaa
      721 cctatattaa gattttacct agttgaatct tcttttctat ataaagcgtc ggagcatatc
      781 agggggttat ctaacgtaaa tgctaccctt cggctcgctt tcgctcggca ttgacgtcag
      841 atactgcacc ccctgaaccc ccatgctcca acagcaa
//
