LOCUS       sequence-150289-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum
            ATCC 824.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren 
            X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
  TITLE     CRISPR-based genome editing and expression control systems in 
            Clostridium acetobutylicum and Clostridium beijerinckii.
  JOURNAL   Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
  PUBMED    27213844
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2016 May 23. doi: 10.1002/biot.201600053."
COMMENT     Sequence Label: pNICKclos1.0
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accttgaact ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta
       61 tttgtgccga tagctaagcc tattgagtat ttcttatcca tcccggggat cctcgacact
      121 cccttttact atttaattat ctatgttaaa tgattaacgt gctcatttat attttaacaa
      181 acttttcaca tgaagttaag ttttttagaa aattatttat attttatact gcagctcgag
      241 gatgtttata cagttactat catgccttgt aatgataaaa aatatgaagc agatattcct
      301 ttcatggaaa ctaacagctt aagagatatt gatgcatcct taactacaag agagcttgca
      361 aaaatgatta aagatgcaaa aattaaattt gcagatcttg aagatggtga agttgatcct
      421 gctatgggta cttacagtgg tgctggagct atctttggtg caaccggtgg cgttatggaa
      481 gctgcaataa gatcagctaa agactttgct gaaaataaag aacttgaaaa tgttgattac
      541 actgaagtaa gaggctttaa aggcataaaa gaagcggaag ttgaaattgc tggaaataaa
      601 ctaaacgttg ctgttataaa tggtgcttct aacttcttcg agtttatgaa atctggaaaa
      661 atgaacgaaa aacaatatca ctttatagaa gtaatggctt gccctggtgg atgtataaat
      721 ggtggaggtc aacctcacgt aaatgctctt gatagagaaa atgttgatta cagaa
//
