LOCUS       sequence-150303-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the endoplasmic reticulum using 
            lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  TITLE     pLV-ER eGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-ER GFP
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..841
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMXXLYK"
     gap             1040..1063
                     /estimated_length=24
     gap             1103..1112
                     /estimated_length=10
ORIGIN
        1 cnnnnnnnnn gnnnnngcag anctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgcccgggcg tacgggatcc caccggtgct
      121 cgagatggtg agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct
      181 gaatggcgat gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac
      241 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc
      301 caccctggtg accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat
      361 gaagcagcac gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat
      421 cttcttcgag gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac
      481 cctggtgaat cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg
      541 caataagatg gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa
      601 gaatggcatc aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct
      661 ggccgaccac taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa
      721 ccactacctg tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat
      781 gatctacttc ggcttcgtga ccgccgccgc catcacccac ggcatggntg anctgtacaa
      841 gtccggactc agatctatgc ctggnccgac ccccagtggc actaangtnn ntcctcnggc
      901 gctctcccag caaagcagtg gccgcccngg ngncnggntc cactgtccgg cagannaant
      961 gccanctgtg nnnaggnngc agncgcanan ncgnngnncg gggggnnnng gcgatnnann
     1021 nnaanatnnn nnggntcaan nnnnnnnnnn nnnnnnnnnn nnntcnnttc nncnnntnnn
     1081 nntnnngtnn nnnnnnggnn agnnnnnnnn nngnnnncna nnnngnnngg nncnannnnn
     1141 ng
//
