LOCUS       sequence-150304-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing gene of interest with 
            Dam-V5 using Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pLgw RFC1-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..167
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(192..197,198..215,216..222)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(342..361)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acaagttacc gagaaagaag aactcacaca cagctagatc aaacaagttt gtacaaaaaa
       61 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
      121 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
      181 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
      241 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      301 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      361 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
      421 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
      481 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
      541 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
      601 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
      661 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
      721 cctgg
//
