LOCUS       sequence-150319-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3-pgk-hph
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acggtatcga taagctatca caagtttgta caaaaaagca ggcttcacca tgtctcatgc
       61 agccgagcca gctcgggacg ccgtagaggc cagcgcggag ggccctcgag ccgtgttcct
      121 gctattggaa gagcgcaggc cagctgagtc ggcccagctg ctcagcctga attctttgct
      181 tccggaatcc gggattgttg ctgatatcga gttagaaaac atcctcgatc ctgacagctt
      241 ctacgagctc aaaagccaac ccctatccct ccgctccagc ctcccaatat cactgcaggc
      301 cacaccgacc accccagcta cactctctgc gtcgtcttct gcagggggct cccggacccc
      361 tgccatgtca tcttcttcat cacgggtctt gctgcgtcag cagcttatga gggcccaggc
      421 tcaggaacag gagaggcgtg agcggcggga acaggcagcg gccgctccct tccccagccc
      481 tgcgcccgcc tcaccagcca tctctgtgat tggcgtgtct gctggtggcc acacactgag
      541 tcgtccacct cctgctcagg tgcccaggga ggtactcaag gttcagaccc acctcgagaa
      601 ccctacacgc taccacctgc agcaagctcg ccggcagcag gtcaaacagt acttgtctac
      661 cacacttggg cccaagctgg cttcccaggc tctcacccca ccaccagggc cttccagtgc
      721 ccagccactt cctgcccctg aaactgctca tgccaccggt cctacaggca gtgctcctaa
      781 cagccccatg gcgctgctca ccattgggtc cagctca
//
