LOCUS       sequence-150330-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent mitophagy reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pCLBW cox8 EGFP mCherry
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(138..848)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(416..435)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     685..703
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(906..927)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
       61 ggttgattat cgataagctt gatatcgaat tctagagtcg cggcctcgaa tcaccacttt
      121 gtacaagaaa gctgggttta cttatacagc tcgtccatgc cgccggtgga gtggcggccc
      181 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      241 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      301 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      361 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      421 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      481 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      541 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      601 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
      661 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
      721 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      781 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
      841 ctcaccatgc ccgagcctga gcccttgtac agctcgtcca tgccgagagt gatcccggcg
      901 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttgggg
//
