LOCUS       sequence-150331-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3::ERT2-pgk-hph
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcctcgacg gtatcgataa gctatcacaa gtttgtacaa aaaagcaggc ttcaccatgt
       61 ctcatgcagc cgagccagct cgggacgccg tagaggccag cgcggagggc cctcgagccg
      121 tgttcctgct attggaagag cgcaggccag ctgagtcggc ccagctgctc agcctgaatt
      181 ctttgcttcc ggaatccggg attgttgctg atatcgagtt agaaaacatc ctcgatcctg
      241 acagcttcta cgagctcaaa agccaacccc tatccctccg ctccagcctc ccaatatcac
      301 tgcaggccac accgaccacc ccagctacac tctctgcgtc gtcttctgca gggggctccc
      361 ggacccctgc catgtcatct tcttcatcac gggtcttgct gcgtcagcag cttatgaggg
      421 cccaggctca ggaacaggag aggcgtgagc ggcgggaaca ggcagcggcc gctcccttcc
      481 ccagccctgc gcccgcctca ccagccatct ctgtgattgg cgtgtctgct ggtggccaca
      541 cactgagtcg tccacctcct gctcaggtgc ccagggaggt actcaaggtt cagacccacc
      601 tcgagaaccc tacacgctac cacctgcagc aagctcgccg gcagcaggtc aaacagtact
      661 tgtctaccac acttgggccc aagctggctt cccaggctct caccccacca ccagggcctt
      721 ccagtgccca gccacttcct gcccctgaaa ctgctcatgc caccggtcct acaggcagtg
      781 ctcctaacag ccccatggcg ctgctcacca ttgggtccag ctca
//
