LOCUS       sequence-150332-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-3xFLAG::Flcn-pgk-hph
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..92
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcctcgacgg tatcgataag cttatggact acaaagacca tgacggtgat tataaagatc
       61 atgacatcga ttacaaggat gacgatgaca agggatcccc catcacaagt ttgtacaaaa
      121 aagcaggctt caccatgaac gccatagtcg ccctctgcca cttctgcgag ctccatggcc
      181 cccgcacgct cttctgcacg gaagttctac acgctcccct gccccagggg gccggaagtg
      241 gggacagtcc tgaccaggtt gagcaggctg aggaggagga gggtggcatt cagatgagca
      301 gccgggtccg tgcccacagc ccagccgagg gtgccagcag tgagtccagc agcccggggc
      361 ccaagaagtc ggacatgtgt gagggctgcc ggtcacttgc cgtagggcac ccaggctata
      421 tcagtcatga taaagagacc tctattaagt acgtcagtca ccagcacccc aaccacccgc
      481 agctcttcag catcgtccgc caggcctgtg tccggagcct gagctgtgag gtatgccctg
      541 gtcgtgaagg ccccatcttc tttggtgatg agcagcacgg ctttgtgttc agccacacct
      601 tcttcatcaa agacagcctg gccagaggct tccagcgctg gtacagcatc atcgccatca
      661 tgatggatcg gatctacctc atcaactcct ggcccttcct gctggg
//
