LOCUS       sequence-150333-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-3xFLAG::Fnip1-pgk-hph
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDY*DHDIDYKDDDDK"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acggtatcga taagcttatg gactacaaag accatgacgg tgattattaa gatcatgaca
       61 tcgattacaa ggatgacgat gacaagggat cccccatcac aagtttgtac aaaaaagcag
      121 gcttcaccat ggcccccacc ctgttccaga agctcttcag caagcggagc ggattaggcg
      181 cgccgggacg agacgcccgc gacccggact gcgcgttcag ttggcccttg ccagaatttg
      241 acccaagtca gattcgactg atcgtatatc aagactgtga aagacgaggg agaaatgtat
      301 tgtttgactc cagtgttaag agaaaaaatg aggacacatc agtatcgaag ctctgtaatg
      361 atgctcaagt caaggtcttt gggaagtgct gccagctgaa gccaggagga gatagttctt
      421 cctctttgga tagctctatc actttatctt ctgatggaaa agaccagtgt cccaaatacc
      481 agggttctcg gtgttcttct gatgccaata tgcttggaga gatgatgttt ggctcagtag
      541 caatgagcta caaaggatcc accttaaaaa ttcatcagat ccgctcgcct ccacagctta
      601 tgcttagcaa agttttcacc gcacggactg gcagtagtat ctgtgggagt ctcaatactc
      661 tgcaggacag tcttgaattc atcaatcagg acagtaatac attgaa
//
