LOCUS       sequence-150349-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the C-terminal fragment of BRCA1 that is endogenously 
            generated by caspase cleavage in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Xu Z, Payoe R, Fahlman RP
  TITLE     The C-terminal proteolytic fragment of the breast cancer 
            susceptibility type 1 protein (BRCA1) is degraded by the N-end rule 
            pathway.
  JOURNAL   J Biol Chem. 2012 Mar 2;287(10):7495-502. doi: 
            10.1074/jbc.M111.301002. Epub 2012 Jan 18.
  PUBMED    22262859
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301002"; journalName: "J Biol Chem"; date: 
            "2012-03-2- 2"; volume: "287"; issue: "10"; pages: "7495-502"
COMMENT     Sequence Label: RF-118-UB-BRCA1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..320
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MEIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ccatggagat cttcgtgaag accctgaccg
      121 gcaagaccat cactctggag gtggagccca gtgacaccat cgaaaatgtg aaagccaaga
      181 tccaagataa agaaggcatc ccccccgacc agcagaggct catctttgca ggcaagcagc
      241 tggaagatgg ccgcactctt tccgactaca acatccagaa agagtcgacc ctgcacctgg
      301 tcctgcgcct gaggggtgga gatggtgaaa taaaggaaga tactagtttt gctgaaaatg
      361 acattaagga aagttctgct gtttttagca aaagcgtcca gagaggagag cttagcagga
      421 gtcctagccc tttcacccat acacatttgg ctcagggtta ccgaagaggg gccaagaaat
      481 tagagtcctc agaagagaac ttatctagtg aggatgaaga gcttccctgc ttccaacact
      541 tgttatttgg taaagtaaac aatatacctt ctcagtctac taggcatagc accgttgcta
      601 ccgagtgtct gtctaagaac acagaggaga atttattatc attgaagaat agcttaaatg
      661 actgcagtaa ccaggtaata ttggcaaagg catctcagga acatcacctt agtgaggaaa
      721 caaaatgttc tgctagcttg ttttcttcac agtgcagtga attggaagac ttgactgcaa
      781 atacaaacac ccaggatcct ttcttgattg gttcttccaa acaaatgagg catcagtctg
      841 aa
//
