LOCUS       sequence-150356-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of C-terminally Flag tagged ATE1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  TITLE     Fahlman lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RF-88-Ate1
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttc accatggctt tctgggcggg gggttcgccc
      121 agcgtcgtgg actatttccc tagcgaggac ttctaccgct gcggctactg caagaacgag
      181 tcgggcagcc gctccaatgg catgtgggca cattccatga cagtacagga ttatcaggat
      241 ctcatagacc gaggatggcg aagaagtgga aaatatgtgt acaaacctgt catgaatcaa
      301 acatgttgtc ctcagtacac aataaggtgc cgacctttac aatttcagcc ttcaaaatct
      361 cacaagaagg ttttgaaaaa aatgttgaaa tttctggcta aaggggaggt tcccaaagga
      421 agttgtgagg atgagcccat ggattccaca atggatgatg ctgttgcggg tgactttgca
      481 ttgataaata aactggatat acagtgtgat cttaaaacac tcagtgatga catcaaagag
      541 agtttagaga gtgaaggaaa aaattcaaag aaagaagaac ctcaggaatt acttcagtca
      601 caagatttcg taggagagaa gttgggctct ggtgaaccgt cacattcagt taaagttcac
      661 acagttccta agccaggcaa aggggctgat ttgagtaagc ctccatgtcg aaaagcaaag
      721 gaaatccgga aagaaaggaa aaggttaaaa ctaatgcagc aga
//
