LOCUS       sequence-150357-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of C-terminally Flag tagged ATE1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  TITLE     Fahlman lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RF-88-Ate1
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..70)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atcacttgtc atcgtcatcc ttgtaatcga tgtcatgatc tttataatca ccgtcatggt
       61 ctttgtagtc gctagccatt ctagactcga ggtttctgaa cagcagcatc cgctcggagc
      121 acttctgccc caccaggctg gcgtactgca gaacagcagc ctcctcactt gggtctttct
      181 gctgtttctt ataaacaccg taaggcatga tggctctctt gtgaaacacc tgcaatcggt
      241 caggttccgt actgcgatcc tcatccactg cttctgggtc ctggttgaaa cggcagtact
      301 tggagttttc aagtgaaggc aggcattgct caatgggtac ccaaacatat gtctcagggc
      361 acagcaaatc agaaggtcta tactgaccct tatatttcat cttgggacac gaatgaatgt
      421 agaaacccat ataataatag ctgagttgag aagttttctc atgaagctgc ctagtaaaag
      481 caatttctcg tagtgcagag tagacgccca aagacaaaaa cgaataatca ggatcgtagt
      541 acaaatacac agatgataca cagtttggga ggatgtcaat cacccccaca gcaatgatct
      601 ttccgtcaag ccagtactgc tggtgaaagg agccatagcc acaatctggc ccattagggg
      661 gagtctctgc ctccaagggt gaactgcaaa ggaatcttgt gaactcagtc ttcccacatt
      721 catcgggtgg atcctggtgt atggccactt gatacttgac atacaa
//
