LOCUS       sequence-150367-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding shRNA and mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pFUChW nontargeting shRNA
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..775)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(343..362)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     612..630
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      772..781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(888..907)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgctttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      121 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
      181 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
      241 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
      301 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
      361 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      421 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      481 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
      541 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
      601 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
      661 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
      721 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg
      781 cgaccggtac tagtctaaca ctgaaaatta catattgacc caaatgatta catttcaaaa
      841 ggtgcctaaa aaacttcaca aaacacactc gccaaccccg agcgcatagt tcaaaaccgg
      901 agcttcagct acttaagaag ataggtacat aaaaccgacc aaagaaactg acgcctcact
      961 tatccctccc ctcaccagag gtccggcgcc tgtcgattca gga
//
