LOCUS       sequence-150377-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac for EOS-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-EOS-GFP-IP
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    303..336
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    375..599
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     588..605
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
       61 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
      121 gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa
      181 aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga taattatatt
      241 gttatttcat gttctactta cgtgataact tattatatat atattttctt gttatagata
      301 tcataacttc gtataatgta tgctatacga agttatgcgg ccctcgaggc tgcaggttct
      361 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
      421 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
      481 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
      541 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
      601 cgaggctgat cagcgagctc tagatcatcg atgcatgggg tcgtgcgctc ctttcggtcg
      661 ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc
      721 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgta
//
