LOCUS       sequence-150391-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_arC9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
       61 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
      121 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
      181 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
      241 tgatcgccca gctgcccggc gcatctccag caaacagtct tgccttgagt ctcacagcag
      301 accagatggt gtcagcactg ctcgatgcgg agccacctat actctactct gaatacgacc
      361 caaccagacc gttcagcgag gcttcaatga tgggactcct gactaatctg gcggacaggg
      421 aacttgtgca catgatcaat tgggccaaac gggttcccgg ttttgttgac cttaccctgc
      481 atgatcaggt tcatctgttg gaatgtgcat ggctcgaaat acttatgatt gggctcgtat
      541 ggaggtccat ggagcaccct ggcaagctcc tgtttgctcc aaatctcttg ttggatcgga
      601 atcagggtaa atgtgtcgaa ggaatggtag agatttttga tatgctcctg gcaacctcc
//
