LOCUS       sequence-150394-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1808_arC9_2xNLS_human
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
       61 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
      121 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
      181 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
      241 cccagctgcc cggcgcatct ccagcaaaca gtcttgcctt gagtctcaca gcagaccaga
      301 tggtgtcagc actgctcgat gcggagccac ctatactcta ctctgaatac gacccaacca
      361 gaccgttcag cgaggcttca atgatgggac tcctgactaa tctggcggac agggaacttg
      421 tgcacatgat caattgggcc aaacgggttc ccggttttgt tgaccttacc ctgcatgatc
      481 aggttcatct gttggaatgt gcatggctcg aaatacttat gattgggctc gtatggaggt
      541 ccatggagca ccctggcaag ctcctgtttg ctccaaatct cttgttggat cggaatcag
//
