LOCUS       sequence-150416-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing gene of interest with 
            Dam-V5 using Gateway cloning .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pLgw EcoDam-V5-RFC1
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..197
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(238..543)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(340..359)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga gtctagaccg
       61 cggttcgatc gaaccacttt gtacaagaaa gctgaacgag aaacgtaaaa tgatataaat
      121 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      181 catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc ctgacattta
      241 tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc
      301 agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg
      361 ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggggactt tatctgacag
      421 cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact
      481 ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg
      541 catttcacca gcccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga
      601 cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt
      661 cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa
      721 ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat acctctt
//
