LOCUS       sequence-150419-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAZ2B
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             472..489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(542..560)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      556..603
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(727..746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat ggatagcaaa gatctggcgc tgtgcagcat
      121 gattctgacc gaaatggaaa cccatgaaga tgcgtggccg tttctgctgc cggtgaacct
      181 gaaactggtt ccgggctata aaaaagtgat taaaaaaccg atggatttta gcaccattcg
      241 tgaaaaactg agcagcggtc agtatccgaa cctggaaacc tttgcgctgg atgtgcgtct
      301 ggtgtttgat aactgcgaaa cctttaacga agatgatagc gatattggcc gtgccggtca
      361 taacatgcgc aaatattttg aaaaaaaatg gaccgatacc tttaaatgac agtaaaggtg
      421 gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      481 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      541 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      601 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
//
