LOCUS       sequence-150420-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAZ2B
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(467..484)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             495..517
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    532..556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(556..575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(557..575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(659..678)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcatttaaag gtatcggtcc
      121 attttttttc aaaatatttg cgcatgttat gaccggcacg gccaatatcg ctatcatctt
      181 cgttaaaggt ttcgcagtta tcaaacacca gacgcacatc cagcgcaaag gtttccaggt
      241 tcggatactg accgctgctc agtttttcac gaatggtgct aaaatccatc ggttttttaa
      301 tcactttttt atagcccgga accagtttca ggttcaccgg cagcagaaac ggccacgcat
      361 cttcatgggt ttccatttcg gtcagaatca tgctgcacag cgccagatct ttgctatcca
      421 tggattggaa gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat
      481 ggtgcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      541 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      601 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      661 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      721 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      781 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      841 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
      901 caaaaccttt cgcggtatgg
//
