LOCUS       sequence-150428-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBS
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(512..532)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(557..574)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             585..607
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    622..646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(646..665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(647..665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(749..768)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tccgtatcca cctttactgt cactcctggg
      121 cggccacgaa gttcagcaag tcaatggcgg tgaccacccc gaacaccatc tgccgctgct
      181 gctcgtgcac caccagggcg aagtggtcca tctccaggat gtgcgagagc ctgcccagcg
      241 tgtccgtgag gcggatctgt ttgaactgct tgtagatgac tttgccaact tggtctgacg
      301 gctgcacctt cccggcaagc agggacgaga gcatgttccc aagcgtcacc attcccagga
      361 ttacccccgc ctcatccacc acgggcgcct ggtcgaagcc cttctcccgg aggatctcga
      421 tggtgtgccc acaggtgatg gtcgggagca cggtcagcgg ggctgacagg cccagctcct
      481 gaacacggag gtgccaccac cagggcttca tggattggaa gtacaggttc tcggtaccca
      541 gatctacacc agaagaatga tgatgatgat ggtgcatatg tatatctcct tcttaaagtt
      601 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      661 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      721 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      781 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      841 gtggccgggg gactgttggg
//
