LOCUS       sequence-150446-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of GST-mR7BD (Rab7 binding domain of murine 
            RILP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Romero Rosales K, Peralta ER, Guenther GG, Wong SY, Edinger AL
  TITLE     Rab7 activation by growth factor withdrawal contributes to the 
            induction of apoptosis.
  JOURNAL   Mol Biol Cell. 2009 Jun;20(12):2831-40. doi: 
            10.1091/mbc.E08-09-0911. Epub 2009 Apr 22.
  PUBMED    19386765
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E08-09-0911"; journalName: "Mol Biol Cell"; date: 
            "2009-06"; volume: "20"; issue: "12"; pages: "2831-40"
COMMENT     Sequence Label: pGEX-4T-3-mR7BD
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(190..195,196..213,214..220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    228..244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     252..268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     252..268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             264..791
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(284..301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(284..300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(293..315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(893..898,899..914,915..921)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    929..945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
       61 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
      181 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      241 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
      301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      421 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
      481 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
      541 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
      601 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
      661 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt agcttatcga
      721 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
      781 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
      841 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
      901 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      961 agtattc
//
