LOCUS       sequence-150455-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP constitutively expressed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
            DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, 
            Regev A, Lander ES, Koonin EV, Zhang F
  TITLE     C2c2 is a single-component programmable RNA-guided RNA-targeting 
            CRISPR effector.
  JOURNAL   Science. 2016 Jun 2. pii: aaf5573.
  PUBMED    27256883
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 2. pii: aaf5573."
COMMENT     Sequence Label: pC007 - pRFP constitutive expression
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     215..232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(265..292)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(308..379)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctgtggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      241 cagctcgagt taggatccta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac
      301 tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
      361 gagcctttcg ttttatttga tgcctggaga tccttattaa gcaccggtgg agtgacgacc
      421 ttcagcacgt tcgtactgtt caacgatggt gtagtcttcg ttgtgggagg tgatgtccag
      481 tttgatgtcg gttttgtaag cacccggcag ctgaaccggt tttttagcca tgtaggtggt
      541 tttaacttca gcgtcgtagt gaccaccgtc tttcagtttc agacgcattt tgatttcacc
      601 tttcagagca ccgtcttccg ggtacatacg ttcggtggaa gcttcccaac ccatggtttt
      661 tttctgcata accggaccgt cggacgggaa gttggtacca cgcagtttaa ctttgtagat
      721 gaactcaccg tcttgcaggg aggagtcctg ggtaacggta acaacaccac cgtcttcgaa
      781 gttcataaca cgttcccatt tgaaaccttc cgggaaggac agtttca
//
