LOCUS       sequence-150461-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible RFP expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
            DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, 
            Regev A, Lander ES, Koonin EV, Zhang F
  TITLE     C2c2 is a single-component programmable RNA-guided RNA-targeting 
            CRISPR effector.
  JOURNAL   Science. 2016 Jun 2. pii: aaf5573.
  PUBMED    27256883
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 2. pii: aaf5573."
COMMENT     Sequence Label: pC008 - pBR322 with tetR-inducible RFP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     839..858
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      301 ggcatgctta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
      361 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
      421 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
      481 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
      541 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
      601 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
      661 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
      721 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
      781 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
      841 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aa
//
