LOCUS       sequence-150462-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible RFP expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
            DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, 
            Regev A, Lander ES, Koonin EV, Zhang F
  TITLE     C2c2 is a single-component programmable RNA-guided RNA-targeting 
            CRISPR effector.
  JOURNAL   Science. 2016 Jun 2. pii: aaf5573.
  PUBMED    27256883
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 2. pii: aaf5573."
COMMENT     Sequence Label: pC008 - pBR322 with tetR-inducible RFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     259..278
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
       61 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      181 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
      241 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac ttaagcaccg
      301 gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg
      361 gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta
      421 gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc
      481 attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc
      541 caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt
      601 ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca
      661 ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc
      721 aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc
      781 ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg
      841 gtctgggtac cttcgtacgg acgaccttca ccttcacctt
//
