LOCUS       sequence-150483-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driven flipped + extended sgRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pJJR50
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(205..554)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     complement(566..582)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(567..583)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(607..626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(608..626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(633..650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(633..649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(878..897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
       61 ggatcccccg ggctgcagga attcgataaa aaaaagcacc gactcggtgc cactttttca
      121 agttgataac ggactagcct tatttaaact tgctatgctg tttccagcat agctcttaaa
      181 ctagtcttcc tctctcgaag acataagaca tctcgcaata ggaggtgttt atataggggc
      241 aacacaagag gcggcagaca tctcgtgagt ttgacagagt agtataaaga gtgtgtgtga
      301 gagggtgtag gcatttcata acatattttt tgtggaataa ttttggaaaa aaggtgtttt
      361 tttaataata gcttttataa gagtttctca aaaattgcat gctttttttg aaaaggttga
      421 caaattatag tagtactaaa gttgctattt tcaggtctca gcagaaaatt cacaactaaa
      481 aataaaattt gcaaaacaga gtgtgcgcct ttaaggtgta cagtttttta ttcctttatt
      541 gctagttttt tttgatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc
      601 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
      661 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      721 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      781 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggg
//
