LOCUS       sequence-150487-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR82
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(142..166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(519..552)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             571..594
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             598..621
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             625..648
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(798..817)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
       61 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
      121 cgtccgaggg caaaggaata gacccagctt tcttgtacaa agtgggtcca attactcttc
      181 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
      241 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
      301 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
      361 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
      421 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
      481 atcatatgtt acgtttcagt ttatgaccgc aatttttaat aacttcgtat agcatacatt
      541 atacgaagtt attttcaggg agccggatct gattataaag acgatgacga taagcgtgac
      601 tacaaggacg acgacgacaa gcgtgattac aaggatgacg atgacaagag actagttcga
      661 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
      721 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
      781 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatg
//
