LOCUS       sequence-150494-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR83
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..156)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(509..542)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             561..584
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             588..611
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             615..638
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
       61 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
      121 caaaggaata gacccagctt tcttgtacaa agtgggtcca attactcttc aacatcccta
      181 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
      241 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
      301 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
      361 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
      421 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
      481 acgtttcagt ttatgaccgc aatttttaat aacttcgtat agcatacatt atacgaagtt
      541 attttcaggg agccggatct gattataaag acgatgacga taagcgtgac tacaaggacg
      601 acgacgacaa gcgtgattac aaggatgacg atgacaagag actagttcga cctgcagact
      661 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
      721 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
      781 cactggccat atcggtggtc at
//
