LOCUS       sequence-150495-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug counter-selectable selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-TKnatAX
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      514..711
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(817..836)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(818..836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catcgaggca ctggatgggt ccttcaccac cgacaccgtc ttccgcgtca ccgccaccgg
       61 ggacggcttc accctgcggg aggtgccggt ggacccgccc ctgaccaagg tgttccccga
      121 cgacgaatcg gacgacgaat cggacgacgg ggaggacggc gacccggact cccggacgtt
      181 cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt actccggctg
      241 gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg ggcacggggt
      301 cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc gagcggggcg ccgggcacct
      361 ctggctggag gtcaccaacg tcaacgcacc ggcgatccac gcgtaccggc ggatggggtt
      421 caccctctgc ggcctggaca ccgccctgta cgacggcacc gcctcggacg gcgagcaggc
      481 gctctacatg agcatgccct gcccctaatc agtactgaca ataaaaagat tcttgttttc
      541 aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt
      601 agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg
      661 cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt
      721 cgatactaac gccgccatcc agtgtttaaa cgagctcgaa ttcatcgatg atatcagatc
      781 cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaat
//
