LOCUS       sequence-150500-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     Boxem lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJJR81
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(145..169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(522..555)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             574..597
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             601..624
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             628..651
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(801..820)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
       61 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
      121 actcgtccga gggcaaagga atagacccag ctttcttgta caaagtgggt ccaattactc
      181 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
      241 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
      301 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
      361 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
      421 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
      481 cttatcatat gttacgtttc agtttatgac cgcaattttt aataacttcg tatagcatac
      541 attatacgaa gttattttca gggagccgga tctgattata aagacgatga cgataagcgt
      601 gactacaagg acgacgacga caagcgtgat tacaaggatg acgatgacaa gagactagtt
      661 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
      721 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      781 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      841 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg
      901 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cac
//
