LOCUS       sequence-150502-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ZCD2 with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Amr S, Heisey C, Zhang M, Xia XJ, Shows KH, Ajlouni K, Pandya A, 
            Satin LS, El-Shanti H, Shiang R
  TITLE     A homozygous mutation in a novel zinc-finger protein, ERIS, is 
            responsible for Wolfram syndrome 2.
  JOURNAL   Am J Hum Genet. 2007 Oct;81(4):673-83. Epub 2007 Aug 20.
  PUBMED    17846994
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet."; date: "2007-10"; volume: "81(4)"; pages: "673-83. Epub 2007
            Aug 20"
COMMENT     Sequence Label: pC-FLAG-ZCD2
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..47)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(599..623)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(633..650)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agggagagag ctatgacgtc tcacttgtcg tcatcgtctt tgtagtcaac cactttgtac
       61 aagaaagctg ggtctagata tctcgaggct acttctttct tcttcagtat tagtggaccc
      121 acattatctc ctgtcaattc attgtgttta ttatgtgaac catcgcaggc aggaaacgtt
      181 ttagaacgcc aacacctaca ataagctgct ttagtaagac acaaatcttc aatgtttatt
      241 tcattcacta ctttcggatt ttccttttgt attttaagat taatcaagct atccttctgt
      301 tgtttcttct tcgggaggaa tggacgaact gcaaggtagc caagaagtgc gagtacacca
      361 aggaaaggca ataaccgaag ccattctgaa actgtgagcc tagcgaaccc ggtaatgctt
      421 tcagggactg ggagccgctt cagatatgca gggagctgca ccttcacgat acgggccacg
      481 ctctccagca ccatcctggc cagcggcgtc cactcctctc ccgagcccgg acgcgtgggt
      541 cgacgatatc ccgggaattc ggtaccggat ccagtcgact gaattggttc ctttaaagag
      601 cctgcttttt tgtacaaact tgttgatcta gagtgatggt gatggtgatg gtagtacgcc
      661 atggtccgcg gcctaggcta gagtccggag gctggatcgg tcccggtgtc ttctatggag
      721 gtcaaa
//
