LOCUS       sequence-150542-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of a Flag-tagged, constitutively active 
            version (S142A, S211A) of Tfeb..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, 
            Dayn Y, Shaw RJ
  TITLE     AMPK governs lineage specification through Tfeb-dependent regulation
            of lysosomes.
  JOURNAL   Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.
  PUBMED    26944679
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.274142"; journalName: "Genes Dev"; date: "2016-03-1- 
            1"; volume: "30"; issue: "5"; pages: "535-52"
COMMENT     Sequence Label: pCIP-Flag-TfebAA
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             72..95
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgggcacgt gctggttatt gtgctgtctc atcattttgg caaagaattc aacgcggcta
       61 gcgccaccat ggattacaag gacgatgacg acaaactcgg atccatggga accaattcag
      121 tcgacgctca gctcgctcag tggtcttggg caaatccctt ctgcccggac tcagtttctc
      181 cttgtgcaca atgggagcaa ccatacttat gccagcctgt gcttaaagac tacgaagatg
      241 atgaatactt catgggcctg tctcccctcg actacaggga gcccgaacca acagctgcca
      301 tggcgtcacg catcgggctg cgcatgcagc tcatgcggga gcaggcccag caggaggagc
      361 agcgagagcg catgcagcag caggctgtca tgcattatat gcaacagcag cagcagcagc
      421 agcagcagct gggtgggccc cccaccccag ccatcaacac ccctgtccac ttccagtcgc
      481 ccccgcctgt gcccggggag gtgctgaagg tgcagtccta cctggagaac cccacctcct
      541 accacctgca acagtcccag catcagaagg ttcgggagta tctgtctgag acctatggga
      601 acaagtttgc tgcccacgtg agcccagccc aaggttcccc gaagcctgcc ccagcagcat
      661 ccccaggggt gcgggctgga cacgtactgt ccacctcggc cggcaacagt gctcccaacg
      721 ctcccatggc catgctacat atcagctcca accccgagaa agagtttgat gatgtcattg
      781 acaacattat gcgcctggac agcgtgctgg gctacatcaa ccctgagatg cagatgccta
      841 acacgctgcc cctgtctagc agc
//
