LOCUS       sequence-150561-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding N-terminal fusion with Venus
            fluorescent protein fragment 1 (V1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-V1-ORF
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(316..774,775..777,778..780))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     455..474
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(887..906)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(888..906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtacctc cagagccacc
      301 atcgatttgc ttgtcggcgg tgatatagac gttgtggctg ttgtagttgt actccagctt
      361 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      421 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      481 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      541 cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagcccaggg tggtcacgag
      601 ggtgggccag ggcacgggca gcttgccggt ggtgcagatc agcttcaggg tcagcttgcc
      661 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      721 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      781 ggtgcggccg ccactgtgct ggatatctgc agaattccac cacactggac tagtggatcc
      841 gagctcggta ccaagcttaa gtttaaacgc tagccagctt gggtctccct atagtgagtc
      901 gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
      961 atatagacct c
//
