LOCUS       sequence-150570-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP fused to the extracellular terminus of 
            neurexin3B. This is a lentiviral plasmid, but can also be used for 
            transfections. Synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPa-NRX in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             346..372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             373..399
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(799..819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agctggtgga ggaaaggacg cctcttccaa ggccaactct ctgggctcta ttatgatggt
       61 ttgaaagtac tgaacatggc ggctgagaac aaccccaata ttaaaatcaa tggaagtgtt
      121 cggctggttg gagaagtccc atcaattttg ggaacaacac agacgacctc catgccacca
      181 gaaatgtcta ctactgtcat ggaaaccact actacaatgg cgactaccac aacccgtaag
      241 aatcgctcta cagccagcat tcagccaaca tcagatgatc ttgtttcatc tgctgaatgt
      301 tcaagtgatg atgaagactt tgttgaatgt gagccgagta cagcataccc atacgatgtt
      361 ccagattacg cttatcctta cgacgtacct gactatgcta accccacgga gccgggaatc
      421 agacgggttc cgggggcctc agaggtgatc cgggagtcga gcagcacaac agggatggtc
      481 gtcggcattg tggctgctgc cgccctctgc atcttgatcc tcctgtacgc catgtacaag
      541 tacaggaaca gggacgaggg gtcctatcaa gtggacgaga cgcggaacta catcagcaac
      601 tccgcccaga gcaacggcac gctcatgaag gagaagcagc agagctcgaa gagcggccac
      661 aagaaacaga aaaacaagga cagggagtat tacgtgtagg tcgacggcgc gcccctgcag
      721 ggaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
      781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      901 gttgctgtct ctt
//
