LOCUS       sequence-150643-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 
            1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, 
            Dayn Y, Shaw RJ
  TITLE     AMPK governs lineage specification through Tfeb-dependent regulation
            of lysosomes.
  JOURNAL   Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.
  PUBMED    26944679
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.274142"; journalName: "Genes Dev"; date: "2016-03-1- 
            1"; volume: "30"; issue: "5"; pages: "535-52"
COMMENT     Sequence Label: Cas9-GFP_sg_mAMPKa1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..143
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        223..298
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        395..680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
       61 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      121 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      181 ttgtggaaag gacgaaacac cgttattgtc acaggcatat gggttttaga gctagaaata
      241 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      301 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc
      361 gccaattctg cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga
      481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      601 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      661 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      721 cccccccctc cccaccccca attttgtatt tatt
//
