LOCUS       sequence-150646-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 
            2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, 
            Dayn Y, Shaw RJ
  TITLE     AMPK governs lineage specification through Tfeb-dependent regulation
            of lysosomes.
  JOURNAL   Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.
  PUBMED    26944679
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.274142"; journalName: "Genes Dev"; date: "2016-03-1- 
            1"; volume: "30"; issue: "5"; pages: "535-52"
COMMENT     Sequence Label: Cas9-GFP_sg_mAMPKa2
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..64)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(80..127)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ccttgctcac tgggccagga ttctcctcga cgtcaccgca tgttagcaga cttcctctgc
       61 cctctccact gccgaattcc tttttctttt ttgcctggcc ggcctttttc gtggccgccg
      121 gccttttgtc gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc
      181 tctggtggat cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga
      241 tggtggtgtc aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt
      301 ggatgatatt ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca
      361 gcactttgtc cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct
      421 cgatgatctc gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat
      481 cctcggggga gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt
      541 tggagggcag ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct
      601 tccggccgtt ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt
      661 tcacttcttt gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc
      721 tttccatgat ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct
      781 tttccacttt ggccaccacc agcacagaat aggccacggt ggggctgtcg aagccgccgt
      841 acttctt
//
