LOCUS       sequence-150651-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: J23114-mRFP1-331Bb
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             57..734
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 ggctagctca gtcctaggta caatgctagc tactagtgaa agaggagaaa tactagatgg
       61 cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg
      121 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc
      181 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt
      241 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact
      301 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg
      361 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag
      421 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg
      481 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca
      541 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca
      601 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca
      661 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact
      721 ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
      781 a
//
