LOCUS       sequence-150656-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-USP19 1-1290 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 1-1290
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        105..123
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     105..122
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgagctc tagcatttag gtgacactat
      121 agaatagggc cctctagatg catgctcgag cggccgctta ccggaggcag ccttcatctg
      181 gtgtggtagg gccacgtggt agggggccca cccagtcttg gggcccccag ggacccaggg
      241 gcccagaccc ctcaggcacc ggctcctcct cagcctccag ctcctgccaa atccgggaag
      301 cctggctggc agcagcctca gctgcagggc ctaggtctgg gtggtgctca gagtgacctg
      361 ccctgggggg cctctccaca ggagagttcc gccggcggta gaagagtaca taggcataac
      421 gcgtcacaac ctggctctcg tctaccgttg tcactgtgct gtcatcaaac aagcgccagc
      481 ccacgtcact gcgctgactg ctacgatcat tgggcaggcg tgcacaggca gtgtagtggc
      541 caccaatcat gcctccatag tggttgatga cagcatatag atcgtagctg ggcagctgct
      601 cctctttctg accaatgcag aacttgctca ggtccaggtt cctaacaggg aactccacca
      661 agtcattgat cttgtcacgc cagataaaac tacgaaagga gaagcgcttg agctgcacga
      721 tgagaacatt tggcaggcgc catagcaaca gctgcttgga ggcctcacgg tgctgtttgc
      781 actgtgggca gtaccaggcc tcctcgggtg ccagcacctc aggccgtgtg aagaggttga
      841 ggcactggtc cagg
//
