LOCUS       sequence-150662-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding N-terminal fusion with Venus
            fluorescent protein fragment 2 (V2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-V2-ORF
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(282..299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    288..512
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      558..986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(645..664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     855..876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(995..1015)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
      241 aaagtggttg tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
      301 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      361 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      421 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      481 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      541 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      781 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      901 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      961 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     1021 aagtc
//
