LOCUS       sequence-150663-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding N-terminal fusion with Venus
            fluorescent protein fragment 2 (V2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-V2-ORF
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(342..363)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(652..671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(716..919)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(745..765)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgtac ctccagagcc accatcgatt
      301 tggtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      361 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg
      421 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      481 agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
      541 ttcatggtgc ggccgccact gtgctggata tctgcagaat tccaccacac tggactagtg
      601 gatccgagct cggtaccaag cttaagttta aacgctagcc agcttgggtc tccctatagt
      661 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
      721 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      781 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      841 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
      901 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
      961 taggaaagtc ccataaggtc atgtactg
//
