LOCUS       sequence-150665-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding C-terminal fusion with Venus
            fluorescent protein fragment 2 (V2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-ORF-V2
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     471..492
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(569..586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    575..799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(887..904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
      241 aaagtggtca tcgatggtgg cggtggctct ggaggtggtg ggtcctccgg aaagaacggc
      301 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
      361 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      421 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      481 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatct
      541 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      601 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      661 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      721 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      781 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgcattaa tgaatcggcc
      841 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      901 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      961 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1021 aggccaggaa ccgtaaaa
//
