LOCUS       sequence-150667-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding C-terminal fusion with Venus
            fluorescent protein fragment 2 (V2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-ORF-V2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(100..106,107..124,125..130))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(155..279)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(339..358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(340..358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(403..606)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(432..452)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        607..986
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
       61 ctcctgaaaa tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
      181 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      241 attttacgtt tctcgttcag cttttttgta caaacttgtc tcgagtaccc cgtaatatgg
      301 taccaagctt aagtttaaac gctagccagc ttgggtctcc ctatagtgag tcgtattaat
      361 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
      421 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
      481 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
      541 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
      601 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
      661 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
      721 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
      781 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
      841 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
      901 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
      961 ttaataacta gtcaataatc aatgtcaacg cgtatatctg
//
