LOCUS       sequence-150675-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing SaCas9 under EFs 
            promoter, gRNAs targeting Dmd introns 22 and 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        357..605
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     357..377
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attagaatta
       61 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
      121 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
      181 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgcag
      241 taatgtgtca taccttcgtt taagtactct gtgctggaaa cagcacagaa tctacttaaa
      301 caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg agattttttt ccgcgggagg
      361 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      421 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      481 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      541 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      601 acaccgatat aatagaaatt attcatgttt aagtactctg tgctggaaac agcacagaat
      661 ctacttaaac aaggcaaaat gccgtgttta tctcgtcaac ttgttggcga gatttttttg
      721 gggatcctct agagtcgagt agataagtag catggcgggt taatcattaa ctacaaggaa
      781 cccctagtga tggagttggc cactccctct ctgcgcgc
//
